DNA base compositions and base sequence relatedness of atypical Campylobacter jejuni strains from clinical material |
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Authors: | R.J. Owen C. Dawson |
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Affiliation: | National Collection of Type Cultures, Central Public Health Laboratory, 61, Colindale Avenue, London, NW9 5HT, U.K. |
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Abstract: | Abstract The relationships of nitrate-negative campylobacters (NNC) resembling Campylobacter jejuni were investigated by DNA base composition estimation (T m method) and DNA-DNA hybridization (S1 endonuclease assay). The 8 NNC strains which were from clinical material formed a homogeneous DNA group with a high level of relatedness (approx. 70%) to typical (nitrate-positive) C. jejuni , but were less similar (approx. 35%) to Campylobacter coli , and only slightly related (≥ 10%) to Campylobacter fetus, Campylobacter laridis and Campylobacter sputorum . The NNC strains showed small but consistent genome differences from typical C. jejuni . As these differences can be correlated with several bacteriological test differences, we conclude that the NNC strains constitute a distinct subspecies within C. jejuni . |
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Keywords: | DNA-DNA hybridization nitrate-negative campylobacters GCLO-2 |
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