三磷酸腺苷对小鼠骨骼肌成纤维细胞NOR-10迁移作用及分子机制初步研究

目的:探讨三磷酸腺苷(ATP)对小鼠骨骼肌成纤维细胞的迁移作用及其可能机制。方法:细胞划痕实验检测1μM、10μM、100μMATP对NOR-10细胞愈合率的影响;细胞迁移小室实验检测空白对照组、100μM ATP组、30μM PPADS+100μM ATP组、100μM RB2+100μM ATP组细胞的迁移率。结果:细胞划痕实验及迁移实验表明高浓度ATP能够促进NOR-10细胞的迁移能力,100μM ATP促细胞迁移能力最强(P0.05),并且其促迁移作用能被30μM PPADS,100μM RB2所抑制(P0.05),但100μM RB2的抑制作用更强(P0.05)。结论:高浓度ATP(10μM)能够促进NOR-10细胞的迁移能力,并且其促迁移可能通过激活P2Y受体作用大于P2X受体。

Effect of ATP on Cell Migration in Mouse Skeletal Muscle FibroblastNOR-10 and Underlying Molecular Mechanism

Objective： To investigate the effect of ATP on mouse skeletal muscle fibroblast cell migration and its possible mechan ism. Methods： NOR-10 fibroblasts healing rate was evaluated under 1 μM, 10 μM, 100 μMATP treatments by cell scratch assay; cell migration rate was estimated by migration chamber in control group, 100 μM ATP group, 30 μM PPADS＋100 μM ATP group, and 100 μM RB2＋ 100μM ATP group. Results： celt scratch and migration chamber experiments indicate that high concentrations of ATP can promote migration of NOR-10 fibroblast, and the capability is strongest by 100 μM ATP treatment （P 〈0.05）, and its role in promoting the migration can be inhibited by either 30 μM PPADS or 100 μM RB2 （P 〈0.05）, but the inhibitory effect is greater by 100 μM RB2 treatment （P 〈0.05）. Conclusion： High concentrations of ATP （〉10 μM） can promote cell migration of NOR-10 fibroblast, which is probably through the activation of P2Y receptors more than P2X receptors.