miR-335对骨肉瘤细胞增殖和迁移的影响

目的:通过研究miR-335对骨肉瘤细胞系SOSP-9607增殖和迁移的影响,探讨miR-335在骨肉瘤细胞生物学行为中的作用。方法:体外培养SOSP-9607细胞并将其分三组,分别为实验组、阴性对照组和空白对照组。实验组转染miR-335模拟物(has-miR-335 mimics),阴性对照组转染阴性对照序列(negative control,NC),空白对照组细胞不行任何转染。采用噻唑蓝(MTT)比色实验法检测和比较细胞处理24、48、72和96 h的增殖情况,采用Transwell实验检测和比较各组细胞的迁移情况。结果:MTT结果显示,实验组细胞48、72和96 h增殖率较阴性对照组明显降低(P0.01),而阴性对照组及空白对照组细胞增殖率比较未见明显差异(P0.05)。在Transwell迁移和侵袭实验中,与阴性对照组比较,实验组细胞的侵袭及迁移能力也明显降低(P0.05),而两对照组细胞迁移及侵袭能力也无明显差异(P0.05)。结论:miR-335可显著抑制骨肉瘤细胞的增殖及迁移,有望成为骨肉瘤治疗的新靶点,值得深入研究。

Effect of miR-335 on the Proliferation and Migration of Osteosarcoma Cells

Objective： To investigate the role of miR-335 in biological behavior of osteosarcoma cells by observing the effect of miR-335 on cell proliferation, migration and invasion of human osteosarcoma cell line SOSP-9607. Methods： SOSP-9607 cells were devided into three groups： experimental group （transfected with miR-335 mimics）, control group （transfected with NC） and blank group （non-transfected）. MTT assay was performed to compare the proliferation ability among groups. We detected the optical density （OD） at 24 h, 48 h, 72 h and 96 h, and then presented the growth curve. Transwell assays were carried out to investigate the migratory and invasive ability of osteosarcoma cells in different groups. Results： In MTT assay, compared with control group, experimental group showed lower cell proliferation of efficiency at 48, 72 and 96 h （P〈0.01）, while no significance difference was shown between control group and blank group. Meantime, in Transwell assays, cells in experimental group showed greater migratory and invasive ability than cells in control group （P〈0.05）, and there was no significance difference between control group and blank group. Conclution： The miR-335 played a significantly repressive role in the proliferation, migration and invasion of osteosarcoma, and may prove to be a novel therapeutic agent.