DJ-1基因siRNA对三阴性乳腺癌细胞侵袭和迁移影响的研究

目的:探讨DJ-1基因siRNA对三阴性乳腺癌细胞体外侵袭和迁移能力的影响。方法:设计DJ-1基因的小分子干扰RNA(siRNA)片段,脂质体介导转染入三阴性乳腺癌细胞株MAD-MB-23l,转染分3个组:A组(空白对照control组)、B组(转染非特异性对照Scramble组)、C组(转染si DJ-1组)。应用Western blotting免疫印迹法检测转染前后DJ-1表达水平;运用细胞迁移和侵袭实验检测细胞迁移和侵袭能力的变化。结果:C组DJ-1蛋白的表达强度弱于A组和B组(t=9.831,P0.05),而A组与B组比较,DJ-1蛋白表达水平则无明显差异(t=1.629,P0.05)。细胞迁移实验中,A组细胞为(218.37±12.75);B组的细胞为(214.46±11.38);C组的细胞为(129.65±8.59),C组细胞明显少于A组和B组(t=10.927,9.984,P0.05),而A组与B组之间,差异无统计学意义(t=0.512,P0.05)。细胞侵袭实验中,A组细胞为(127.28±12.65);B组的细胞为(123.06±13.08);C组的细胞为(52.85±9.58),C组穿过人工基底膜的细胞明显少于A组和B组(t=7.927,8.643,P0.05),而A组与B组之间,差异无统计学意义(t=0.627,P0.05)。结论:DJ-1基因siRNA可抑制三阴性乳腺癌细胞侵袭和迁移。

Influence of RNA Interference Induced Silencing of DJ-1 Gene on Migration and Invasion Potential in Triple-Negative Breast Cancer Cells

Objective： To investigute the effect of RNA interference induced silencing ofDJ-1 gene on migration and invasion potential in triple-negative breast cancer cells. Methods： Human breast cancer cells MAD-MB-231 were divided into control group（A group）, Scramble group（B group） and si DJ-1 group（C group）, siRNA sequence targeting DJ-1 gene were designed and synthesized. The siRNA were transfected into triple-negative breast cancer cell by lipofectamine TM 2000 mediation. The alteration of DJ-1 protein expression was detected by western blotting, Invasion potential were evaluated by transwell invasion assay, Cell migrating ability was detected by transwell migration assay. Results： Transfection of D J-1 siRNA significantly knocked down the D J-1 protein expression, The migration and invasion of breast cancer cells declined after transfection with siRNA DJ-1, DJ-1 protein expression of C group was lower than that of A group and B group （t=-9.831, P〈0.05）. There was no difference between A group and B group （t=-1.629, P〉0.05）. In cell migration assay, the number of cells in C group （129.65± 8.59） was less than that in A group （218.37± 12.75） and B group （214.46±11.38） （t=10.927, 9.984, P〈0.05）. There was no difference between A group and B group （t=-0.512, P〉0.05）. In cell migration assay, the number of cells in C group（129.65± 8.59） was less than that in A group（218.37± 12.75） and B group（214.46± 1.38） （t=10.927, 9.984, P〈0.05）. There was no difference between A group and B group （t=0.512, P〉0.05）. In invasion potential test, the number of cells in C group（52.85± 9.58） was less than that in A group （127.28± 12.65） and B group （123.06± 13.08） （t=7.927, 8.643, P〈0.05）. There were no difference between A group and B group （t=0.627, P〉0.05）. Conclusions： The siRNA targeting D J-1 gene can restrain the migration and invasion of triple-negative breast cancer cell.