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Modified AFLP analysis method for species with small genomes
Authors:Celia?M.?James  author-information"  >  author-information__contact u-icon-before"  >  mailto:celia.james@hri.ac.uk"   title="  celia.james@hri.ac.uk"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author,Silke?S.?Lesemann,Graeme?J.?Down
Affiliation:(1) Horticulture Research International, East Malling, ME19 6BJ West Malling, Kent, UK;(2) Federal Centre for Breeding Research on Cultivated Plants, Institute of Fruit Breeding, Pillnitzer Plantz 3a, D-01326 Dresden, Germany
Abstract:A modified method for detecting amplified fragment length polymorphism (AFLP) has been developed for species with small genomes. We successfully used a combination of primers with a reduced number of selective bases to differentiate and fingerprint isolates ofColletotrichum acutatum, the causative agent of strawberry black spot, an important strawberry disease.
Keywords:amplified fragment length polymorphism   Colletotrichum acutatum   diversity  fingerprinting
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