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Modified AFLP analysis method for species with small genomes
Authors:Email author" target="_blank">Celia?M?JamesEmail author  Silke?S?Lesemann  Graeme?J?Down
Institution:(1) Horticulture Research International, East Malling, ME19 6BJ West Malling, Kent, UK;(2) Federal Centre for Breeding Research on Cultivated Plants, Institute of Fruit Breeding, Pillnitzer Plantz 3a, D-01326 Dresden, Germany
Abstract:A modified method for detecting amplified fragment length polymorphism (AFLP) has been developed for species with small genomes. We successfully used a combination of primers with a reduced number of selective bases to differentiate and fingerprint isolates ofColletotrichum acutatum, the causative agent of strawberry black spot, an important strawberry disease.
Keywords:amplified fragment length polymorphism            Colletotrichum acutatum            diversity  fingerprinting
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