Development and characterization of microsatellite markers for parentage analyses of the coral reef damselfish (<Emphasis Type="Italic">Pomacentrus amboinensis</Emphasis>: Pomacentridae) |
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Authors: | Katherine P Munkres Line K Bay Dean R Jerry Mark I McCormick Lynne Van Herwerden |
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Institution: | (1) School of Marine Biology and Aquaculture, James Cook University, Townsville, QLD, 4811, Australia;(2) School of Tropical Biology, James Cook University, Townsville, QLD, 4811, Australia |
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Abstract: | Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles
per locus ranging from 6–23 and observed heterozygosity between 0.42–0.92. In addition, the cross-species testing of six primers
developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (≥14 alleles) in at least four of the six loci and a high proportion of tetranucleotide
repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual
relatedness. |
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Keywords: | Damselfish Microsatellite Pomacentridae Coral reef Genetic diversity |
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