Inhibition of tobacco mosaic virus replication in lateral roots is dependent on an activated meristem-derived signal |
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Authors: | Valentine T A Roberts I M Oparka K J |
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Institution: | (1) Unit of Cell Biology, Scottish Crop Research Institute, Dundee, GB |
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Abstract: | Summary. Viral invasion of the root system of Nicotiana benthamiana was studied noninvasively with a tobacco mosaic virus (TMV) vector expressing the green-fluorescent protein (GFP). Lateral
root primordia, which developed from the pericycle of primary roots, became heavily infected as they emerged from the root
cortex. However, following emergence, a progressive wave of viral inhibition occurred that originated in the lateral-root
meristem and progressed towards its base. Excision of source and sink tissues suggested that the inhibition of virus replication
was brought about by the basipetal movement of a root meristem signal. When infected plants were inoculated with tobacco rattle
virus (TRV) expressing the red-fluorescent protein, DsRed, TRV entered the lateral roots and suppressed the host response,
leading to a reestablishment of TMV infection in lateral roots. By infecting GFP-expressing transgenic plants with TMV carrying
the complementary GFP sequence it was possible to silence the host GFP, leading to the complete loss of fluorescence in lateral roots. The data suggest that viral inhibition in lateral roots occurs
by a gene-silencing-like mechanism that is dependent on the activation of a lateral-root meristem.
Received July 23, 2001 Accepted October 11, 2001 |
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Keywords: | : Tobacco mosaic virus Nicotiana benthamiana Root Meristem Viral suppression Green-fluorescent protein |
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