Escherichia coli isolated from seafood: toxicity and plasmid profiles |
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Authors: | Grace Teophilo Regine dos Fernandes Vieira Dália dos Prazeres Rodrigues Francisca R Menezes |
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Institution: | Instituto de Ciências do Mar-Labomar, Universidade Federal do Ceará, Meireles, Fortaleza, Brazil. |
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Abstract: | Thirty-two Escherichia coli strains were isolated from red snapper (Lutjanus purpureus) and from seabob shrimp (Xiphopenaeus kroyeri). The strains were numbered S1–S16, and F1–F16, which corresponds to the isolation origin from shrimp (S) and fish (F). The
isolates were biologically and antigenically characterized by agglutination tests with enteropathogenic E. coli (EPEC)-, enteroinvasive E. coli (EIEC)- and enterohemorrhagic E. coli (EHEC) O157-specific antisera. The ETEC enterotoxins were characterized by GMI-ELISA for enterotoxin LT-1 (thermolabile)
and by inoculation of supernatants prepared from newly born mice for enterotoxin Sta. A total of 14 strains produced exotoxins,
of which seven were thermolabile (LT) and seven were thermostable (ST). Antimicrobial susceptibility profiles were determined
by disc diffusion in agar using ampicillin, cephalothin, cefoxitin, ceftriaxone, imipenem, nalidixic acid, ciprofloxacin,
chloramphenicol, gentamicin, nitrofurantoin, sulfamethoxazole–trimethoprim, and tetracycline. Four isolates showed lower susceptibility
to some antibiotics, two strains were resistant to ampicillin, tetracycline, and sulfamethoxazole-trimethoprim, and two were
resistant to tetracycline and nitrofurantoin. Plasmids were extracted in the four resistant isolates; two of them contained
plasmids whose molecular weight varied from low to high. The characterization of LT- and ST-toxin-producing E. coli strains displaying multiresistance and containing plasmids suggests the need for tightening current control measures for the
use of antimicrobials.
Electronic Publication |
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Keywords: | Escherichia coli Enterotoxigenicity Seabob shrimp (Xiphopenaeus kroyeri) Red snapper fish (Lutjanus purpureus) Antimicrobial resistance |
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