刺五加注射液对豚鼠庆大霉素耳毒性拮抗作用的实验研究

目的：探讨中药刺五加注射液（ASS）对庆大霉素（GM）耳毒性作用的影响及其机制。方法：豚鼠随机分成对照组、GM组、ASS4-GM组和ASS组。采用听性脑干反应（ABR）、透射电镜技术（TEM）、Western blot方法观察用药前后豚鼠的ABR阈值、形态学变化及caspase-3的表达情况。结果：用药后GM组ABR阈值明显升高;ASS组ABR阈值与对照组相比无显著性差异,与GM组和ASS4-GM组相比明显降低。透射电镜观察用药后GM组毛细胞损伤严重,出现了凋亡的形态学特征,而ASS4-GM组损伤较轻。Western blot结果表明用药后GM组豚鼠caspase-3表达明显增加;ASS4-GM组caspase-3的表达稍有升高。结论：刺五加注射液对庆大霉素耳毒性具有拮抗作用,其机制可能是通过抑制caspase-3的表达来实现的。

Inhibitive effect of acanthopanax senticosus injection on gentamicin-induced ototoxicity in guinea pigs

Aim: To study the antagonistic action of acanthopanax senticosus injection(ASS) on gentamicin ototoxicity.Methods: Guinea pigs were randomly divided into control group,GM group,ASS group,and ASS+GM group.The changes of hearing threshold,cochlear morphology,expression of caspases-3 were determined by ABR,TEM,and Western blot,respectively.Results: The ABR threshold in GM group increased markedly.There was no significant difference in ABR threshold between ASS group and control group,but the ABR threshold in ASS group was much lower than that both in GM group and ASS+GM group.Severely injured hair cells with morphological characteristics of apoptosis were found under TEM in GM group,and the hair cells were less injured in ASS+GM group.The results of Western blot showed that the expression of caspase-3 increased markedly in GM group,but it increased slightly in ASS+GM group.Conclusion: ASS may antagonize the GM ototoxicity by inhibiting the expression of caspases-3.