Effects of alkylating agents on lymphocytes from controls and from patients with Fanconi's anemia |
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| Authors: | Bo?ena Novotná Petr Goetz Nadě?da I Surkova |
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| Institution: | (1) Department for Congenital Defects, Institute of Experimental Medicine, Czechoslovak Academy of Sciences,  robárova 50, Prague 10, Czechoslovakia;(2) Genetic Department of the Research Institute for Child Development, Motol, V úvalu 84, Prague 5, Czechoslovakia;(3) The Scientific Research Laboratory of Experimental Biologic Models, Academy of Medical Sciences USSR, Moscow |
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| Abstract: | Summary The frequency of sister chromatid exchanges (SCE) and chromosome aberrations and the dynamics of cell division in peripheral blood lymphocytes of four patients with Fanconi's anemia were studied after in vitro exposure to alkylating agents TEPA and mitomycin.SCE frequency was significantly increased even after very low doses of mutagens, while chromosome aberrations were significantly increased only after high doses (0.160  g/ml mitomycin and 10-5
M TEPA). The responses of Fanconi's anemia cells and control cells did not differ significantly. The increased frequency of both SCE and chromosome aberrations was accompanied by gradual delay of cell division, which was most conspicuous in cells from patients with Fanconi's anemia. |
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