Partial characterization of prostaglandin synthetase in the reproductive tract of the male house cricket, |
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Authors: | Don B. Destephano U. Eugene Brady Leesa B. Woodall |
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Affiliation: | Department of Entomology, University of Georgia Athens, Georgia 30602 USA |
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Abstract: | An active prostaglandin (PG) synthetase was found in the 12100 g pellet of reproductive tract homogenates of the male house cricket, . Comparatively, the 12100 g supernatant and the microsomal fractions were inactive. The PG synthetase in the pellet fraction was characterized in terms of cofactor, temperature, pH, and incubation time requirements. Indomethacin, a known inhibitor of mammalian PG synthetase, was not inhibitory to the cricket synthetase. The procedure and findings are relevant to PG synthetase studies of any organism or tissue. |
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