Decreased regulatory T‐cells and CD4+/CD8+ ratio correlate with disease onset and progression in patients with generalized vitiligo |
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Authors: | Mitesh Dwivedi Naresh C Laddha Prateek Arora Yogesh S Marfatia Rasheedunnisa Begum |
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Institution: | 1. Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, , Vadodara, India;2. Department of Skin and VD, Sir Sayajirao Gaikwad Medical College, The Maharaja Sayajirao University of Baroda, , Vadodara, India |
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Abstract: | The aim of present study was to evaluate CD4+/CD8+ ratio and CD4+CD25hiFoxP3+ Tregs in GV patients with reference to their effect on disease onset and progression. Flow cytometry was used for determination of CD4+/CD8+ ratio and Tregs in 82 patients and 50 controls. CD8+ T‐cell counts were significantly higher in GV patients as compared with controls (p = 0.003). Active GV patients showed higher CD8+ T‐cell counts compared with stable GV patients (p = 0.001). The CD4+/CD8+ ratio decreased significantly in patients as compared with controls (p = 0.001). Moreover, the ratio in active GV patients significantly lowered as compared with stable GV patients (p = 0.002). Significant decrease in Treg cell percentage and counts in GV patients was observed compared with controls (p = 0.009, p = 0.008) with significant reduction in FoxP3 expression (p = 0.024). Treg cell percentage and counts were significantly decreased in active GV patients compared with stable GV patients (p = 0.007, p = 0.002). Our results suggest that an imbalance of CD4+/CD8+ ratio and natural Tregs in frequency and function might be involved in the T‐cell mediated pathogenesis of GV and its progression. |
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Keywords: | generalized vitiligo CD4+/CD8+ ratio CD4+CD25hiFoxP3+ regulatory T‐cells (Tregs) FoxP3 expression melanocyte autoimmunity |
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