Species composition and arthropod pest feeding of phytoseiid mites in a Japanese pear greenhouse |
| |
| Authors: | Hisato Ishii Yuya Mikawa Yuko Murase Shoji Sonoda Norihide Hinomoto Hidenari Kishimoto Shingo Toyoshima Masatoshi Toyama |
| |
| Affiliation: | 1.School of Agriculture,Utsunomiya University,Utsunomiya,Japan;2.Central Region Agricultural Research Center,NARO,Tsukuba,Japan;3.Apple Research Station, Institute of Fruit Tree and Tea Science,NARO,Morioka,Japan;4.Kanaya Tea Research Station, Institute of Fruit Tree and Tea Science,NARO,Shizuoka,Japan;5.Tsukuba Headquarter, Institute of Fruit Tree and Tea Science,NARO,Tsukuba,Japan |
| |
| Abstract: | A population survey of spider mites and phytoseiid mites was conducted on Japanese pear leaves in a greenhouse. For the survey, the method to estimate phytoseiid mite species composition using quantitative sequencing was modified to be applicable for phytoseiid mite species inhabiting in the greenhouse. Results show the dominant appearance of Neoseiulus californicus (McGregor), Neoseiulus womersleyi (Schicha), and Neoseiulus makuwa (Ehara) from the end of June to late September and their contribution in spider mite control. PCR-based method to detect the ribosomal internal transcribed spacer (ITS) sequences of spider mites from phytoseiid mites was developed. The method shows sensitivity to detect the ITS sequences of Tetranychus urticae Koch from single N. californicus adult at 168 h after ingestion of the spider mite. PCR-based method to detect the cytochrome c oxidase subunit I sequences of several arthropod pests belonging to Hemiptera, Thysanoptera, and Acari from phytoseiid mites was also developed. Results show that phytoseiid mites prey on Eriophyes chibaensis (Kadono) and Aphis gossypii (Glover), in addition to spider mites. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
