Sterol metabolism: XXXVII. On the oxidation of cholesterol by dioxygenases |
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Authors: | Jon I Teng Leland L Smith |
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Institution: | Division of Biochemistry, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77550 USA |
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Abstract: | The oxidation of cholesterol by plant and mammalian dioxygenases yielding cholesterol 7α- and 7β-hydroperoxides has been demonstrated. Cholesterol oxidation is coupled to the oxygenation of polyunsaturated fatty acid esters by soybean lipoxygenase, to the reduction of hydrogen peroxide catalyzed by horseradish peroxidase, and to the oxidation of NADPH by the NADPH-dependent microsomal lipid peroxidation system of rat liver. The initially formed epimeric cholesterol 7-hydroperoxides are transformed in each case to the commonly encountered corresponding 7-alcohol and 7-ketone derivatives. These dioxygenase transformations thus mimic in detail the radiation-induced free radical oxidation of cholesterol by molecular oxygen. Electronically excited (singlet) molecular oxygen is not implicated in these transformations. |
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