Cu2+-catalyzed peptide bond formation in the reaction of 5-deoxypyridoxal and α-phenyl-α-aminomalonic acid

The reaction of 5-deoxypyridoxal with α-phenyl-α-aminomalonic acid in the presence of excess Cu²⁺ ions is shown to lead to the formation of N-5-deoxypyridoxoyl-α-phenylglycine, III, as the only 5-deoxypyridoxal-derived product. This reaction occurs anaerobically under very mild conditions of temperature and pH and involves the oxidative formation of a peptide bond. It represents a hitherto undescribed reaction type for vitamin B₆ and its analogs; a mechanism for the reaction is proposed.