Absence of gas vesicle protein in a mutant of Anabaena flos-aquae |
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Authors: | A E Walsby |
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Institution: | (1) Marine Science Laboratories, Menai Bridge, LL59 5EH Anglesey, Wales, UK |
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Abstract: | Filaments without gas vacuoles arose spontaneously in the gas-vacuolate alga Anabaena flos-aquae. The non-vacuolate mutant was enriched by repeated sedimentation and subsequently cloned by microsyringe transfer. No revertants have been observed. In the gas-vacuolate wild-type alga the gas vesicle protein was clearly distinguished by gel electrophoresis as one of the ten most abundant protein species present in whole cell extracts. Electrophoresis indicated that the mutant had lost the ability to synthesize the gas vesicle protein. A second mutant partially defective in production of gas vacuoles and gas vesicle protein has been isolated.Abbreviations gv
gas vesicle protein
- pb
phycobilin
- TCA
trichloracetic acid |
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Keywords: | Gas vacuoleless mutant Gas vesicle protein Blue-green alga Anabaena flos-aquae |
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