Relationship Between Oxidation of Glutathione and Reactive Nitrogen Species During the Early-Reperfusion Phase of Cerebral Ischemia |
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Authors: | Iijima Takehiko Sakamoto Hideaki Okada Chikako Iwao Yasuhide |
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Institution: | (1) Department of Anesthesiology, Kyorin University, School of Medicine, Mitaka, Japan |
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Abstract: | A timed profile of glutathione oxidation and reactive nitrogen species during reperfusion after cerebral ischemia in rat was obtained. Dialysate was collected every 25 min from a microdialysis probe inserted into the cerebral cortex before and after cerebral ischemia. NO2
–, NO3
–, and reduced and oxidized glutathione (GSH, GSSG) were detected by high-performance liquid chromatography. GSH and GSSG increased and reached a peak: 3408 ± 1710% (mean ± SE) at 25 min of reperfusion (P < 0.0001) and 329 ± 104% at 50 min of reperfusion (P = 0.06), respectively. Oxidation ratio decreased from 0.82 ± 0.04 to 0.42 ± 0.07 (P < 0.0001) at 25 min of reperfusion. NO3
– levels significantly decreased (68.3 ± 9.1%) (P < 0.01) during ischemia and remained lower than the control value during reperfusion. NO2
– levels did not significantly change. These data suggest that GSH releases during early phase of reperfusion and that its rapid oxidation contributes to prevent an increase in reactive nitrogen species. |
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Keywords: | Glutathione nitrate nitrite ischemia free radical reactive nitrogen species |
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