In vitro recurrent selection of potato: production and characterization of salt tolerant cell lines and plants |
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Authors: | Ochatt SJ Marconi PL Radice S Arnozis PA Caso OH |
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Institution: | (1) Lab Physiologie & Culture in Vitro, INRA, URGAP, BV 1540, 21034 Dijon Cedex, France;(2) Centro de Ecofisiología Vegetal (, CONICET, Serrano 669, 1414 Buenos Aires, Argentina |
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Abstract: | A stable salt-tolerant potato cell line, able to grow on media containing 60–450 mM NaCl (i.e. low to high salinity) was selected.
Callus grown on 120 or 150 mM NaCl showed higher fresh weights than the rest of the treatments. Replacing NaCl by KCl or Na2SO4
showed that reductions in fresh weight were mainly due to the presence of Na+ ions. When PEG 6000 was added to the medium
instead of salt, the salt tolerant cell lines were unable to overcome the PEG-induced water stress. Whole plants, regenerated
from salt tolerant callus, exhibited salt stress tolerance as evidenced by their higher fresh and dry weights when watered
with 90 mM NaCl, and they also produced more tubers per plant under salt stress. Salt-tolerant plants differed phenotypically
from control plants both in terms of leaf shape, tuber flesh and skin colour, which was reddish. In addition, DNA fingerprinting
by RAPDs, with 70 different primers, confirmed that the salt tolerant regenerants also differed genotypically from the control,
salt sensitive Kennebec potato plants from which they had been selected.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Na+ tolerance plant regeneration salt stress Solanum tuberosum somaclonal variation RAPDs |
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