NMR of molecules interacting with lipids in small unilamellar vesicles |
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Authors: | Grégory Da Costa Liza Mouret Soizic Chevance Elisabeth Le Rumeur Arnaud Bondon |
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Institution: | (1) RMN-Interactions Lipides Protéines, UMR CNRS 6026, IFR 140, PRISM, Université de Rennes 1, 35043 Rennes Cedex, France |
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Abstract: | Detailed characterization of protein, peptide or drug interactions with natural membrane is still a challenge. This review
focuses on the use of nuclear magnetic resonance (NMR) for the analysis of interaction of molecules with small unilamellar
vesicles (SUV). These phospholipid vesicles are often used as model membranes for fluorescence or circular dichroism experiments.
The various NMR approaches for studying molecule-lipid association are reviewed. After a brief survey of the SUV characterization,
the use of heteronuclear NMR (phosphorous, carbon, fluorine) is described. Applications of proton NMR through transferred
nuclear Overhauser effect to perform structural determination of peptide are presented. Special care is finally given to the
influence of the kinetic of the interactions for the proton NMR of bound molecules in SUV, which can constitute a good model
for the study of dynamical processes at the membrane surface.
Presented at the joint biannual meeting of the SFB-GEIMM-GRIP, Anglet France, 14–19 October, 2006. |
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