Physiological and genetic characterization of rice nitrogen fixer PGPR isolated from rhizosphere soils of different crops |
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Authors: | Safiullah Habibi Salem Djedidi Kunlayakorn Prongjunthuek Md Firoz Mortuza Naoko Ohkama-Ohtsu Hitoshi Sekimoto Tadashi Yokoyoma |
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Affiliation: | 1. United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan 2. Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan 3. Soil Microbiology Research Group, Soil Science Division, Department of Agriculture, Bangkok, Thailand 4. Institute of Food and Radiation Biology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh 5. Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan 6. Faculty of Agriculture, Utsunomiya University, Utsunomiya, Japan
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Abstract: | Aims We aimed to identify plant growth-promoting rhizobacteria that could be used to develop a biofertilizer for rice. Methods To obtain plant growth-promoting rhizobacteria, rhizosphere soils from different crops (rice, wheat, oats, crabgrass, maize, ryegrass, and sweet potato) were inoculated to rice plants. In total, 166 different bacteria were isolated and their plant growth-promoting traits were evaluated in terms of colony morphology, indole-3-acetic acid production, acetylene reduction activity, and phosphate solubilization activity. Moreover, genetic analysis was carried out to evaluate their phylogenetic relationships based on 16S rRNA sequence data. Results Strains of Bacillus altitudinis, Pseudomonas monteilii, and Pseudomonas mandelii formed associations with rice plants and fixed nitrogen. A strain of Rhizobium daejeonense showed nitrogen fixation activity in an in vitro assay and in vivo. Strains of B. altitudinis and R. daejeonense derived from rice rhizosphere soil, strains of P. monteilii and Enterobacter cloacae derived from wheat rhizosphere soil, and a strain of Bacillus pumilus derived from maize rhizosphere soil significantly promoted rice plant growth. Conclusions These methods are effective to identify candidate species that could be developed as biofertilizers for target crops. |
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