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A tracking marker for the first dimension of the two-dimensional gel electrophoresis of ribosomal proteins
Authors:D P Leader
Institution:Department of Chemistry, Texas Christian University, Forth Worth, TX 76129, U.S.A.
Abstract:Protein content of different subcellular fractions from chick brain is compared by using Lowry, TCA—Lowry and Bradford assay methods. Caution is urged in application of Bradford's method to general assay for protein concentration in subcellular fractions.
Keywords:Lowry assay  Bradford assay  protein  subcellular fractions  myelin  carbonic anhydrase  TCA  trichloroacetic acid  BSA  bovine serum albumin  BGG  bovine gamma globulin  SPM  synaptic plasma membrane
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