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Multiple Parameters Drive the Efficiency of CRISPR/Cas9-Induced Gene Modifications in Yarrowia lipolytica |
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| Authors: | Vinciane Borsenberger Djamila Onésime Delphine Lestrade Coraline Rigouin Cécile Neuvéglise Fayza Daboussi Florence Bordes |
| Institution: | 1. LISBP, Université de Toulouse, INSA, INRA, CNRS, Toulouse, France;2. Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, Paris, France;3. TWB, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France |
| Abstract: | |
| Keywords: | oleaginous yeast genome editing sgRNA processing sequencing sgRNA single guide RNA DSB double-strand break RNP ribonucleic acid protein PAM protospacer adjacent sequence NHEJ non-homologous end-joining tRNA transfer RNA Pol III polymerase III KO knockout WT wild-type HDR homology-directed repair |
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