|
|||||
|
|
Plasmodium chabaudi: Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp. |
|
| Authors: | Rubina L. Caldeira,Tiago M. Martins,Carlos Novo,Virgí lio do Rosá rio |
| Affiliation: | a INETI-UTPAM - National Institute of Engineering, Technology and Innovation, Unit of Protein and Monoclonal Antidody Technology, Estrada do Paço do Lumiar, 22, 1649-038 Lisboa, Portugal b iMed-UL - Research Institute for Medicines and Pharmaceutical Sciences, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal c IBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal d IHMT-CMDT - Institute of Hygiene and Tropical Medicine, Center of Malaria and Tropical Diseases, Rua da Junqueira, 96, 1349-008 Lisboa, Portugal |
| Abstract: | Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction.Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity. |
| Keywords: | Malaria Plasmodium chabaudi Recombinant cysteine proteases Chabaupain |
| 本文献已被 ScienceDirect 等数据库收录！ | |