Babesia bovis: Effect of Albumax II and orotic acid in a low-serum in vitro culture |
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Authors: | Claudia Sánchez Elisa Campos Abel G Oliva |
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Institution: | Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Biomolecular Diagnostics Laboratory, Av. da República (EAN), Apt. 127, 2781-901 Oeiras, Portugal |
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Abstract: | Bovine serum is an essential factor for the continuous in vitro growth of Babesia bovis parasites. Culture media typically contain 40% (v/v) of bovine serum. In the present study assays with low-serum media were performed. The growth of B. bovis Mo7 was successively lower in media with 30%, 20% and, principally, with 10% of serum. Without serum, the parasites were not able to propagate. In media with 10% of serum, the supplementation with Albumax™ II improved visibly the growth of B. bovis at the end of each cycle. Regarding the addition of orotic acid, no considerable effect was observed in media with 20% or 10% of serum, with or without Albumax™ II. B. bovis parasites cultured in vitro in all these media maintain their typical morphology during the intraerythrocytic stages. |
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Keywords: | Babesia bovis In vitro culture Low-serum medium Albumax&trade II Orotic acid |
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