Hypoxia alters contractile protein homeostasis in L6 myotubes |
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Authors: | Marc-André Caron |
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Affiliation: | Centre de recherche de l’ Institut Universitaire de Cardiologie et de Pneumologie de l’Université Laval, 2725 Chemin Ste-Foy, Quebec City, QC, Canada, G1V 4G5 |
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Abstract: | Since hypoxia might contribute to the development of muscle atrophy, we wished to provide direct evidence linking hypoxia to muscle atrophy. By evaluating protein degradation and synthesis in hypoxic myotubes we found a significant reduction in total protein content. Using functional assays we observed protein degradation elevation in the first 24 h while synthesis was maintained during this period and then significantly decrease at 48 h. These results demonstrate a temporal regulation of protein homeostasis, whereby elevated protein degradation is followed by a reduction in synthesis. These results are comparable to the cellular adaptation seen during development of muscle atrophy. |
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Keywords: | COPD, chronic obstructive pulmonary disease Ub-P&rsquo some, ubiquitin-proteasome DMEM, Dulbecco&rsquo s modified Eagle&rsquo s medium E3, ubiquitin ligase EDTA, ethylenediamine tetraacetic acid GSK-3, glycogen synthase kinase-3 IGF-1, insulin-like growth factor-1 PBS, phosphate buffered saline PI3K, phosphatidylinositol-3 kinase |
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