Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells |
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| Authors: | Atsuko Masumi Isao Hamaguchi Madoka Kuramitsu Takuo Mizukami Kazuya Takizawa Haruka Momose Seishiro Naito Kazunari Yamaguchi |
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| Affiliation: | Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan |
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| Abstract: | Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-γ treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states. |
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| Keywords: | IRF, interferon regulatory factor HSC, hematopoietic stem cell 5-FU, 5-fluorouracil CFU-Mk, colony forming unit-megakaryocyte CFU-GM, CFU-granulocyte/macrophage EGFP, enhanced green fluorescent protein TPO, thrombopoietin MN cells, mononuclear cells ISRE, interferon-stimulated response element |
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