The PsbP protein, but not the PsbQ protein, is required for normal thylakoid architecture in Arabidopsis thaliana |
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Authors: | Xiaoping Yi |
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Institution: | Division of Biochemistry and Molecular Biology, Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA |
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Abstract: | Interfering RNA was used to suppress the expression of the genes At1g06680 and At2g30790 in Arabidopsis thaliana, which encode the PsbP-1 and PsbP-2 proteins, respectively, of Photosystem II. A phenotypic series of transgenic plants was recovered that expressed intermediate and low amounts of PsbP. Earlier we had documented significant alterations in a variety of Photosystem II parameters in these plant lines Yi, X., Liu, H., Hargett, S. R., Frankel, L. K., Bricker, T. M. (2007). The PsbP protein is required for photosystem II complex assembly/stability and photoautotrophy in Arabidopsis thaliana. J. Biol. Chem. 34, 24833-24841]. In this communication, we document extensive defects in the thylakoid membrane architecture of these plants. Interestingly, strong interfering RNA suppression of the genes encoding the PsbQ protein (At4g21280 and At4g05180) was found to have no effect on the architecture of thylakoid membranes. |
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Keywords: | DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea MS medium Murashigi and Skoog medium PS I Photosystem I PS II Photosystem II LiDS-PAGE lithium dodecyl sulfate polyacrylamide gel electrophoresis |
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