Schistosoma mansoni: Developmental arrest of miracidia treated with histone deacetylase inhibitors |
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Authors: | A Azzi |
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Institution: | Parasitologie Fonctionnelle et Evolutive, UMR 5244 CNRS/Université de Perpignan/EPHE, 52, Avenue Paul Alduy, 66860 Perpignan, France |
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Abstract: | In the present study, we examined the effect of the histone deacetylase (HDAC) inhibitors trichostatin A (TSA), valproic acid (VA) and sodium-butyrate on the metamorphosis of larvae of the human blood-fluke Schistosoma mansoni from the free-swimming miracidia into the intramolluskal sporocyst. We show that HDAC inhibitors block transformation in concentration dependant manner. TSA reversibly blocks this developmental process: only 13 ± 11% of TSA treated miracidia transform into sporocysts in-vitro, compared to 92 ± 3% in the mock-treated control. Other enzyme inhibitors such as cycloheximide or hydroxyurea had no effect on metamorphosis. For treatment of up to 4 h, the effect of TSA was completely reversible. Our data indicates that HDAC activity is necessary for the transformation of S. mansoni miracidia during infection of the snail host. |
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Keywords: | Platyhelminth Schistosoma mansoni Histone deacetylase Trichostatin A Metamorphosis Development |
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