Glycoproteomics: Past, present and future |
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Authors: | Bé rangè re Tissot,Alessio Ceroni,Maria Panico,Antonietta Capone,Anne Dell,Howard R. Morris |
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Affiliation: | a Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom b Department of Evolutionary Biology, University of Siena, Via A. Moro 2, 53100 Siena, Italy c M-SCAN Ltd., Millars Business Centre, Wokingham, Berks, RG41 2TZ, United Kingdom |
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Abstract: | This invited paper reviews the study of protein glycosylation, commonly known as glycoproteomics, beginning with the origins of the subject area in the early 1970s shortly after mass spectrometry was first applied to protein sequencing. We go on to describe current analytical approaches to glycoproteomic analyses, with exemplar projects presented in the form of the complex story of human glycodelin and the characterisation of blood group H eptitopes on the O-glycans of gp273 from Unio elongatulus. Finally, we present an update on the latest progress in the field of automated and semi-automated interpretation and annotation of these data in the form of GlycoWorkBench, a powerful informatics tool that provides valuable assistance in unravelling the complexities of glycoproteomic studies. |
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Keywords: | conA, concanavalin A dHex, deoxyhexose ES, electrospray Fuc, fucose Gal, galactose GalNAc, N-acetylgalactosamine GC, gas chromatography Glc, glucose GlcNAc, N-acetylglucosamine Hex, hexose HexNAc, N-acetylhexosamine LC, liquid chromatography Man, mannose MALDI, matrix-assisted laser desorption ionisation NeuAc, N-acetylneuraminic acid MS, mass spectrometry MS/MS, tandem mass spectrometry NMR, nuclear magnetic resonance POMC, pro-opiomelanocortin PTM, post-translational modification Q-TOF, quadrupole orthogonal acceleration time of flight TOF, time of flight TIC, total ion chromatogram VVL, Vicia villosa lectin |
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