|
|||||
|
|
Down-regulation of adenine nucleotide translocase 3 and its role in camptothecin-induced apoptosis in human hepatoma QGY7703 cells |
|
| Authors: | Zhenlin Hu Xueqing Guo Qi Yu Jianzhong Li Cheng Guo Junping Zhang |
| Affiliation: | a Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China b State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China c Department of Pharmacy, Sixth Affiliated People Hospital, Shanghai Jiaotong University, Shanghai 200233, China |
| Abstract: | Adenine nucleotide translocase (ANT) is known as a core component of the mitochondrial permeability transition pore (MPTP) and a key player in cell death. However, its role in camptothecin (CPT)-induced apoptosis has not been examined. We showed that CPT-induced apoptosis in QGY7703 cells and down-regulated the expression of ANT3. Using ANT3 knock-out and overexpression experiments, we provide further evidence that ANT3 plays a contributive role in CPT-induced apoptosis through induction of MPTP. We speculate that the down-regulation of ANT3 upon stimulation with CPT may be part of the molecular basis underlying the mechanism of acquired resistance to CPT. |
| Keywords: | ANT, adenine nucleotide translocase CPT, camptothecin MPTP, mitochondrial permeability transition pore ΔΨm, mitochondrial membrane potential |
| 本文献已被 ScienceDirect 等数据库收录！ | |