Wave expansion of CD34 progenitor cells in the spleen in rodent malaria |
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Authors: | Felipe Pessoa de Melo Hermida Daniel Perez Vieira Elaine Raniero Fernandes |
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Institution: | a Instituto de Ciências Biomédicas da Universidade de São Paulo (ICB-USP) Professor Lineu Prestes Avenue, Cidade Universitária, São Paulo, SP 05508-900, Brazil b Instituto de Pesquisas Energéticas e Nucleares da Comissão Nacional de Energia Nuclear (IPEN-CNEN/USP) 2242 Professor Lineu Prestes Avenue, Cidade Universitária, São Paulo, SP 05508-000, Brazil c Laboratório de Patologia de Moléstias Transmissíveis da Faculdade de Medicina da Universidade de São Paulo (FMUSP) 455 Doutor Arnaldo Avenue, Cerqueira César, São Paulo, SP 01246903, Brazil d Laboratório de Protozoologia do Instituto de Medicida Tropical de São Paulo (IMTSP-USP) 470 Doutor Enéas de Carvalho Aguiar Avenue, Cerqueira César, São Paulo, SP 05403-000, Brazil |
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Abstract: | Defense against malaria depends upon amplification of the spleen structure and function for the clearance of parasitized red blood cells (pRBC). We studied the distribution and amount of CD34+ cells in the spleens of mice infected with rodent malaria. We sought to identify these cells in the spleen and determine their relationship to infection. C57BL/6J mice were infected with self-resolving, Plasmodium chabaudi CR, or one of the lethal rodent malaria strains, P. chabaudi AJ and P. berghei ANKA. We then recorded parasitemia, mortality, and the presence of CD34+ cells in spleen, as determined by immunohistochemistry and flow cytometry. In the non-lethal strain, the spleen structure was maintained during amplification, but disrupted in lethal models. The abundance of CD34+ cells increased in the red pulp on the 4th and 6th days p.i. in all models, and subsided on the 8th day p.i. Faint CD34+ staining on the 8th day p.i., was probably due to differentiation of committed cell lineages. In this work, increase of spleen CD34+ cells did not correlate with infection control. |
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Keywords: | Rodent malaria Plasmodium chabaudi Plasmodium berghei Spleen CD34+ cells |
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