The prion or the related Shadoo protein is required for early mouse embryogenesis |
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Authors: | Rachel Young,Bruno Passet,Marthe Vilotte,Vincent Bé ringue,Hubert Laude,Jean-Luc Vilotte |
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Affiliation: | a INRA-UMR1313, Génétique Animale et Biologie Intégrative, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France b INRA-UR892, Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France |
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Abstract: | The prion protein PrP has a key role in transmissible spongiform encephalopathies but its biological function remains largely unknown. Recently, a related protein, Shadoo, was discovered. Its biological properties and brain distribution partially overlap that of PrP. We report that the Shadoo-encoding gene knockdown in PrP-knockout mouse embryos results in a lethal phenotype, occurring between E8 and E11, not observed on the wild-type genetic background. It reveals that these two proteins play a shared, crucial role in mammalian embryogenesis, explaining the lack of severe phenotype in PrP-knockout mammals, an appreciable step towards deciphering the biological role of this protein family. |
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Keywords: | Prion Shadoo RNA interference Mouse Embryo Transgenesis |
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