The prion or the related Shadoo protein is required for early mouse embryogenesis |
| |
Authors: | Rachel Young Bruno Passet Marthe Vilotte Vincent Béringue Hubert Laude Jean-Luc Vilotte |
| |
Institution: | a INRA-UMR1313, Génétique Animale et Biologie Intégrative, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France b INRA-UR892, Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France |
| |
Abstract: | The prion protein PrP has a key role in transmissible spongiform encephalopathies but its biological function remains largely unknown. Recently, a related protein, Shadoo, was discovered. Its biological properties and brain distribution partially overlap that of PrP. We report that the Shadoo-encoding gene knockdown in PrP-knockout mouse embryos results in a lethal phenotype, occurring between E8 and E11, not observed on the wild-type genetic background. It reveals that these two proteins play a shared, crucial role in mammalian embryogenesis, explaining the lack of severe phenotype in PrP-knockout mammals, an appreciable step towards deciphering the biological role of this protein family. |
| |
Keywords: | Prion Shadoo RNA interference Mouse Embryo Transgenesis |
本文献已被 ScienceDirect 等数据库收录! |
|