|
|||||
|
|
Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase |
|
| Authors: | Natalia A. Belikova,Ashley Glumac,Ruslan Rafikov,Jianfei Jiang,Joel S. Greenberger,Hü lya Bayir |
| Affiliation: | a Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA b Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 15201, USA c Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA d Department of Radiation Oncology, University of Pittsburgh, Pittsburgh, PA 15260, USA |
| Abstract: | Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H2O2 increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD.Structured summaryMINT-7288408: MnSOD (uniprotkb:P04179) and MnSOD (uniprotkb:P04179) physically interact (MI:0915) by zymography (MI:0512) |
| Keywords: | H2O2, hydrogen peroxide PI, propidium iodide PS, phosphatidylserine AA, antimycin A MnSOD, manganese superoxide dismutase IR, ionizing radiation MECs, mouse embryonic cells SC, scrambled control |
| 本文献已被 ScienceDirect 等数据库收录！ | |