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Babesia bigemina: In vitro multiplication of sporokinetes in Ixodes scapularis (IDE8) cells |
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| Authors: | Múcio FB Ribeiro Camila V Bastos Lygia MF Passos |
| Institution: | a Departamento de Parasitologia, ICB-UFMG, Belo Horizonte, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, Minas Gerais, Brazil b Institute for Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Munich, Leopoldstr. 5, 80802 Munich, Germany c Departamento de Medicina Veterinária Preventiva, Escola de Veterinária - UFMG, Belo Horizonte, Minas Gerais, Brazil |
| Abstract: | This paper describes the in vitro multiplication process of Babesia bigemina sporokinetes in a cell line (IDE8) from Ixodes scapularis ticks. The inoculum was obtained from hemolymph of engorged females of Rhipicephalus (Boophilus) microplus ticks naturally infected with B. bigemina. These ticks had been fed on calves living in a tick endemic farm in Brazil. Microscopic morphological details are shown to describe the development of the parasite in the tick cells; the identity of the parasite was confirmed by a duplex PCR method. |
| Keywords: | Babesia bigemina IDE8 cells In vitro culture |
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