Duddingtonia flagrans: centrifugal flotation technique with magnesium sulphate for the quantification and qualification of chlamydospores in sheep faeces |
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Authors: | Silva Aleksandro Schafer da Zanette Régis Adriel Otto Mateus Anderson Soares Cláudia Dal Molin Alves Sydney Hartz Monteiro Silvia Gonzalez Santurio Janio Morais |
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Institution: | Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil |
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Abstract: | Duddingtonia flagrans is a nematode-trapping fungus responsible for attacking larval stages of helminths in pasture, which has potential as a biological control method. The aim of this study was to test the magnesium sulphate centrifugal flotation technique for the quantification of D. flagrans chlamydospores in sheep faeces and to verify their morphological viability. In this experiment one sheep received an oral dose of 4.5 × 106 chlamydospores/day during 20 days. Fecal samples were collected between days 15 and 20 and analyzed by the centrifugal flotation technique with magnesium sulphate. Densities of 1.23, 1.27 and 1.31 g mL−1 recovered 1.45 × 105, 3.87 × 105 and 1.65 × 105 chlamydospores from the faeces, respectively. Based upon the results it was concluded that this is an efficient technique for the chlamydospores quantification in ovine faeces. Moreover, it allowed more accurate visualization of chlamydospore morphology. |
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Keywords: | Duddingtonia flagrans Chlamydospores Biological control |
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