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Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells |
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| Authors: | Jung-Eun Gil Joong-Hyun Shim Hyun-Ju You Sun Ha Paek Jong-Hoon Kim |
| Institution: | a Laboratory of Stem Cell Biology, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea b Department of Pathology, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea c Department of Neurosurgery, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea |
| Abstract: | We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs. |
| Keywords: | hESCs human embryonic stem cells VN vitronectin Shh sonic hedgehog hEBs human embryoid bodies RA retinoic acid GRM glial restriction medium bFGF fibroblast growth factor PDGF platelet-derived growth factor IGF-I insulin-like growth factor-I cAMP cyclic adenosine 3&prime 5&prime -monophosphate PCR polymerase chain reaction GAPDH glyceraldehyde-3-phosphate dehydrogenase PBS phosphate buffered saline PFA paraformaldehyde DAPI 4&prime 6-diamidino-2-phenylindole OPCs oligodendrocyte precursor cells |
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