|
|||||
|
|
Two-dimensional pulsed electron spin resonance characterization of N-labeled archaeal Rieske-type ferredoxin |
|
| Authors: | Toshio Iwasaki Rimma I Samoilova Sergei A Dikanov |
| Institution: | a Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Tokyo 113-8602, Japan b Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Novosibirsk 630090, Russia c Department of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA |
| Abstract: | Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type 2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems. |
| Keywords: | ARF archaeal Rieske-type ferredoxin from Sulfolobus solfataricus ENDOR electron-nuclear double resonance EPR electron paramagnetic resonance ESEEM electron spin-echo envelope modulation FT Fourier transform Em redox potential HF hyperfine HYSCORE hyperfine sublevel correlation NMR nuclear magnetic resonance Np peptide backbone nitrogen SDX sulredoxin (a high-potential Rieske protein from Sulfolobus tokodaii) T4moC Rieske-type ferredoxin component of toluene 4-monooxygenase complex |
| 本文献已被 ScienceDirect 等数据库收录！ | |