The antifungal plant defensin RsAFP2 from radish induces apoptosis in a metacaspase independent way in Candida albicans |
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Authors: | An M Aerts Sophie Lefevre Isabelle EJA François Renata Santos Karin Thevissen |
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Institution: | a Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium b Institute of Molecular Biosciences (IMB), Karl-Franzens University Graz, Humboldtstraße 50/EG, 8010 Graz, Austria c Institut Jacques Monod, Mitochondria, Metals and Oxidative Stress Laboratory UMR7592-CNRS and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris cedex 13, France |
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Abstract: | We show that the antifungal plant defensin Raphanus sativus antifungal protein 2 (RsAFP2) from radish induces apoptosis and concomitantly triggers activation of caspases or caspase-like proteases in the human pathogen Candida albicans. Furthermore, we demonstrate that deletion of C. albicans metacaspase 1, encoding the only reported (putative) caspase in C. albicans, significantly affects caspase activation by the apoptotic stimulus acetic acid, but not by RsAFP2. To our knowledge, this is the first report on the induction of apoptosis with concomitant caspase activation by a defensin in this pathogen. Moreover, our data point to the existence of at least two different types of caspases or caspase-like proteases in C. albicans. |
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Keywords: | CaMCA1 Candida albicans metacaspase 1 CFU colony forming unit DHE dihydroethidium GlcCer glucosylceramide PI propidium iodide PS phosphatidylserine ROS reactive oxygen species RsAFP2 Raphanus sativus antifungal protein 2 TUNEL terminal transferase-mediated dUTP nick end labeling z-VAD-FMK carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone |
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