Over-expressed microRNA-27a and 27b influence fat accumulation and cell proliferation during rat hepatic stellate cell activation |
| |
| Authors: | Juling Ji Jinsheng Zhang Guangcun Huang Jin Qian |
| |
| Institution: | a Department of Pathology, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, Shanghai 200032, PR China
b Department of Pathology, College of Medicine, Nantong University, Nantong 226001, PR China |
| |
| Abstract: | Hepatic stellate cells (HSCs) activation is an initial event in liver fibrosis. MicroRNAs (miRNAs) have been found to play essential roles in cell differentiation, proliferation, and fat metabolism. In this study, we showed that down-regulation of two over-expressed miRNAs, miR-27a and 27b allowed culture-activated rat HSCs to switch to a more quiescent HSC phenotype, with restored cytoplasmic lipid droplets and decreased cell proliferation. Mechanistically, retinoid X receptor α was confirmed to be the target of miR-27a and 27b. These results indicated a new role and mechanism of miR-27a and 27b in regulating fat metabolism and cell proliferation during HSCs activation. |
| |
| Keywords: | α-SMA α-smooth muscle actin BrdU bromodeoxyuridine HSCs hepatic stellate cells LI labeling index miRNA microRNA MRE microRNA recognition element RXRα retinoid X receptor α 3&prime UTR 3&prime untranslated region |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
