Apoptosis signal-regulating kinase 1 regulates the expression of caspase-11 |
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Authors: | Jong-Ryoul Choi Hyejung Heo Yoo Lang Shin Jung Kang |
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Affiliation: | a Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea b Department of Biology, Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea |
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Abstract: | Caspase-11 is an inducible caspase involved in the regulation of cell death and inflammation. In the present study, we examined whether apoptosis signal-regulating kinase 1 (Ask1)-mediated signaling pathway is involved in the expression of caspase-11 induced by lipopolysaccharide (LPS). We found that the induction of caspase-11 was suppressed by the inhibitors of NADPH oxidase (Nox) or knockdown of Nox4 that acts downstream of toll-like receptor 4 and generates Ask1-activating reactive oxygen species. Overexpression of dominant negative tumor necrosis factor receptor associate factor 6 also suppressed the induction of caspase-11. Importantly, knockdown or dominant negative form of Ask1 suppressed the induction of caspase-11 following LPS stimulation. Taken together, our results show that Ask1 regulates the expression of caspase-11 following LPS stimulation. |
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Keywords: | Ask1 Caspase-11 LPS Nox4 TLR4 |
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