Development of Schistosoma mansoni in the laboratory rat analyzed by light and confocal laser scanning microscopy |
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| Authors: | F.W. Silva-Leitã o,R.H. Neves |
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| Affiliation: | a Laboratory of Helminthology Romero Lascasas Porto, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Biomedical Center, Rua Prof. Manoel de Abreu 444/50 andar, Vila Isabel, 20511-070 Rio de Janeiro, Brazil
b Laboratory of Helminths Parasites of Vertebrates, Department of Helminthology, Oswaldo Cruz Institute, Av. Brasil 4365, Manguinhos, 21045-900 Rio de Janeiro, Brazil |
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| Abstract: | The kinetic of maturation (schistogram) of Schistosoma mansoni worms grown in laboratory rats was studied by light and confocal laser scanning microscopy. Infected rats with the BH strain were weekly euthanized 3-9 weeks pi. Recovered flukes stained with hydrochloric carmine were preserved as whole-mounts and analyzed by confocal and brightfield microscopy. Worms displayed varying degrees of maturation of the reproductive system at weeks 3-6. Male worms showed complete maturation of the reproductive system at week 6, while female worms completed their maturation at week 7. Males presented few tubercles in tegument in all weeks. Despite the presence of a developing embryo within the ootype, no uterine egg was found. The schistogram in rats follows a pattern similar to that observed in mice hosts. |
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| Keywords: | Schistosoma mansoni Rattus norvegicus White rat Confocal microscopy Schistogram |
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