Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum |
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Authors: | Jürgen Manfred Knott |
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Institution: | Institut für Phytopathologie, Universität Kiel, Hermann-Rodewald-Straße 9, D-24118 Kiel, Germany |
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Abstract: | Polyamines are ubiquitously present in all organisms. In addition to the common polyamines, thermophilic archaea synthesize long-chain polyamines. In the present study polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum were cloned and their substrate specificity was analyzed. The polyamine synthase HbSpeE II from H. butylicus synthesized long-chain polyamines with high activity using the same mechanism that is used by a wide range of organisms to synthesize common polyamines, in which the aminopropyl residue derives from decarboxylated S-adenosylmethionine. This is the first polyamine synthase described that synthesizes a polyamine longer than a tetramine with high activity. |
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Keywords: | Long-chain polyamine Archaea Polyamine synthesis Hyperthermus butylicus Pyrobaculum aerophilum |
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