A cold-induced thioredoxin h of rice, OsTrx23, negatively regulates kinase activities of OsMPK3 and OsMPK6 in vitro |
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| Authors: | Guosheng Xie Hideki Kato Kentaro Sasaki |
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| Institution: | a Crop Cold Tolerance Team, National Agricultural Research Center for Hokkaido Region, National Agricultural and Food Research Organization, Sapporo 062-8555, Japan
b National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | Cytosolic thioredoxins are small conserved proteins that are involved in cellular redox regulation. Here, we report that a major and cold-induced thioredoxin h of rice, OsTrx23, has an inhibitory activity on stress-activated mitogen-activated protein kinases (MAPKs), OsMPK3 and OsMPK6 in vitro. This inhibition effects were redox-dependent and did not involve stable physical interaction. The data suggested a novel mechanism for redox regulation of MAPKs in plants.Structured summaryMINT-7234362: MPK3 (uniprotkb:Q10N20) phosphorylates (MI:0217) MBP (uniprotkb:P02687) by protein kinase assay (MI:0424)MINT-7234435: MPK6 (uniprotkb:Q336X9) phosphorylates (MI:0217) MBP (uniprotkb:P02687) by protein kinase assay (MI:0424) |
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| Keywords: | NADP-MDH NADP-Malate Dehydrogenase GST glutathione S-transferase IPTG isopropyl-β-D-thiogalactopyranoside MAPK Mitogen-activated protein kinase MBP Myelin basic protein NTR NADPH-dependent thioredoxin reductase PBS phosphate-buffered saline TBS Tris-buffered saline |
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