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Current trends in high throughput proteomics in cyanobacteria |
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| Authors: | Saw Yen Ow |
| Institution: | Biological and Environmental Systems Group, ChELSI, Department of Chemical and Process Engineering, The University of Sheffield, Sheffield S1 3JD, UK |
| Abstract: | Advancements in genome sequencing and high throughput proteomics of cyanobacterial strains led to 13 published reports, from a small number of laboratories. These successful studies focused on Synechocystis, Nostoc and Anabaena strains, prochlorococcus, and halotolerant Euhalothece. The implications of emerging quantitative aspects developed and applied in these large-scale studies are assessed in the wake of advanced cyanobacterial research. Furthermore, contributions from traditional and early high throughput analysis of cyanobacterial proteomics are compared and summarised. Finally, opinions are provided to link both the trends and the future challenges. This review aims to push the synergy between proteomics and cyanobacterial research to improve both the technical and biological significance. |
| Keywords: | HTT high throughput techniques MS mass spectrometry or tandem mass spectrometry MS/MS fragment peptide scan LC liquid chromatography 2DGE two-dimensional gel electrophoresis 1DGE one-dimensional gel electrophoresis MALDI matrix assisted laser desorption ionisation MALDI-TOF/TOF MALDI interface-tandem time-of-flight analyser Qq-TOF electrospray quadrupole time-of-flight analyser Q-IT electrospray quadrupole ion trap analyser LC-MALDI liquid chromatography MALDI spotting interface m/z mass over charge ratio ICAT isotope coded affinity tags iTRAQ isobaric tags for relative and absolute quantification |
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