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Ankrd17, an ubiquitously expressed ankyrin factor, is essential for the vascular integrity during embryogenesis |
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| Authors: | Shin-Chen Hou Li-Wei Chan Ching-Yuan Su Yen-Ling Shih C-K James Shen Yu-Ting Yan |
| Institution: | a Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC b Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC c Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Zhu Nan Town, Miaoli, Taiwan, ROC d Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC |
| Abstract: | Ankyrin repeat domain 17 (Ankrd17) encodes an ubiquitously expressed protein with two clusters of ankyrin repeats. We have used gene targeting strategy to ablate the Ankrd17 gene in mouse. The Ankrd17-deficient mice died between embryonic day (E) 10.5 and E11.5 due to cardiovascular defects. Serious hemorrhages were detected and the vascular smooth muscle cells (vSMCs) surrounding the vessels were drastically reduced in the Ankrd17-deficient embryos, suggesting that the vascular maturation was not completed. Interestingly, vSMC differentiation marker genes were up-regulated in the mutant embryos. Our data have demonstrated the indispensability of Ankrd17 functioning for vascular maturation during early development. The Ankrd17-deficient mice also provide a new animal model for the analysis of the regulatory pathways of the differentiation of vSMC precursor cells. |
| Keywords: | Ankyrin factor Mouse gene targeting Vascular integrity SM α-actin gene Smooth muscle cell |
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