Pleiotropic roles of calumenin (calu-1), a calcium-binding ER luminal protein, in Caenorhabditis elegans |
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Authors: | Jeong Hoon Cho Hyun-Ok Song Gunasekaran Singaravelu Hyun Sung Soonjae Kwon Joohong Ahnn |
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Affiliation: | a Division of Biology Education, College of Education, Chosun University, Gwangju 501-759, Republic of Korea b Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea c Department of Life Science, BK21 (Life Science for Global Warming Team), College of Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea |
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Abstract: | Calumenin is a Ca2+ binding protein localizing at the lumen of the endoplasmic reticulum (ER). Although it has been implicated in various diseases, the in vivo functions of calumenin are largely unknown. Here, we report that calumenin has pleiotropic roles in muscle and cuticle function in Caenorhabditis elegans. Mutant analysis revealed that the calu-1 is required for regulating fertility, locomotion and body size. In addition, calu-1 is important for two behaviors, defecation and pharyngeal pumping, consistent with its ability to bind Ca2+. The genetic analysis further suggested the possibility that calu-1 regulates the pharyngeal pumping together with the inositol 1,4,5-triphosphate (IP3) receptor encoded by itr-1. Taken together, our data suggest that calumenin is important for calcium signaling pathways in C. elegans. |
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Keywords: | ER, endoplasmic reticulum IPTG, isopropyl β- smallcaps" >d-1-thiogalactopyranoside SERCA, sarcoplasmic reticulum Ca2+-transporting ATPase HIV, human immunodeficiency virus pBoc, posterior body contraction SEM, scanning electron microscopy RNAi, RNA interference IP3, inositol 1,4,5-triphosphate PDI, protein disulfide isomerase PPIase, peptidyl prolyl cis-trans isomerase |
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