Differences between human lymphocyte subpopulations with respect to the uptake and incorporation of [3H]uridine and ribonucleoside triphosphate pools. |
| |
Authors: | M Staub M Sasvári-Székely T Spasokukotskaja F Antoni K Merétey |
| |
Affiliation: | 1. GESAAF, University of Florence, Via Donizetti, 6 50144 Firenze, Italy;2. TasteMatters Research & Consulting, Sydney, Australia |
| |
Abstract: | Cellular [3H]uridine uptake and incorporation into RNA were compared in human tonsil lymphocyte subpopulations obtained by separation on nylon-wool column. While the nonadherent subpopulation, Fraction I, contained 74.5 ± 8% E-rosette forming T-lymphocytes, the adherent fraction, Fraction II, contained 6.5 ± 3.5% of the same cells.The B-cell-enriched Fraction II of lymphocytes, freshly prepared from tonsils, was 2.5-3 times as active as Fraction I with respect to both uptake and incorporation of [3H]uridine. However, in phytohaemagglutinin-stimulated cultures the T-cell-enriched Fraction I proved to be more active with regard to 3H-uridine incorporation.The size of the four ribonucleoside triphosphate pools determined by the RNA-polymerase test was practically the same in both lymphocyte subpopulations. The radioactivity of the acid soluble cell fraction after [3H]uridine labeling consisted of about 60% [3H]UTP in both B- and T-cell-rich lymphocyte fractions, as determined by chromatography on PEI cellulose.On the basis of these results it seems unlikely that [3H] uridine labeling is a specific marker of either of the lymphocyte subpopulations. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|