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The Hortega Silver Carbonate Method for Staining Pituicytes After Paraffin Embedding
Authors:William M. Shanklin
Affiliation: a Department of Histology, School of Medicine, American University of Beirut, Beirut, Lebanon
Abstract:In three papers published on pituicytes, of the ox by Bucy (1930), of the human by Shanklin (1940), and of the horse by Vazquez-Lopez (1942) the pituitaries were sectioned by the freezing method and stained by the Hortega silver carbonate technic. Since that time, as a routine procedure in our laboratory, frozen sections have been replaced by paraffin which in no way interferes with the Hortega silver carbonate staining.
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